Whole genome amplification: methods and protocols /
Whole genome amplification: methods and protocols /
edited by Thomas Kroneis, Institute of Cell Biology, Histology & Embryology, Medical University of Graz, Graz, Austria.
- New York Humana Press 2015
- x, 284 pages : illustrations (some color) ; 27 cm.
- Springer protocols, 1949-2448 Methods in molecular biology, v. 1347 1064-3745 ; .
- Springer protocols (Series), Methods in molecular biology (Clifton, N.J.) ; v. 1347, .
Includes bibliographical references and index.
Principles of whole-genome amplification / Bias in whole genome amplification : causes and considerations / Single-cell lab or how to perform single-cell molecular analysis / Sample preparation methods following cellsearch approach compatible of single-cell whole-genome amplification : an overview / Deterministic whole-genome amplification of single cells / Construction of a DNA library on microbeads using whole genome amplification / Heat-induced fragmentation and adapter-assisted whole genome amplification using GenomePlex® single-cell whole genome amplification kit (WGA4) / Whole genome amplification by isothermal multiple strand displacement using Phi29 DNA polymerase / Using multiplex PCR for assessing the quality of whole genome amplified DNA / Quality control of isothermal amplified DNA based on short tandem repeat analysis / Laser microdissection of FFPE tissue areas and subsequent whole genome amplification by Ampli1 / Whole genome amplification from blood spot samples / Analysis of whole mitogenomes from ancient samples / Copy number variation analysis by array analysis of single cells following whole genome amplification / Whole genome amplification in genomic analysis of single circulating tumor cells / Whole genome amplification of labeled viable single cells suited for array-comparative genomic hybridization / Low-volume on-chip single-cell whole genome amplification for multiple subsequent analyses / Detection and characterization of circulating tumor cells by the cellsearch approach / Zbigniew Tadeusz Czyz, Stefan Kirsch, and Bernhard Polzer -- Jeffrey Sabina and John H. Leamon -- Roland Kirchner and Marianna Alunni-Fabbroni -- Joost F. Swennenhuis and Leon Terstappen -- Zbigniew Tadeusz Czyż and Christoph A. Klein -- Takaaki Kojima, Bo Zhu, and Hideo Nakano -- Amin El-Heliebi, Shukun Chen, and Thomas Kroneis -- Thomas Kroneis and Amin El-Heliebi -- Amin El-Heliebi, Shukun Chen, and Thomas Kroneis -- Thomas Kroneis and Amin El-Heliebi -- Zbigniew Tadeusz Czyz, Nikolas H. Stoecklein, and Bernhard Polzer -- Karina Meden Sørensen -- Gloria Gonzales Fortes and Johanna L.A. Paijmans -- Eftychia Dimitriadou, Masoud Zamani Esteki, and Joris Robert Vermeesch -- Christin Gasch, Klaus Pantel, and Sabine Riethdorf -- Thomas Kroneis and Amin El-Heliebi -- Thomas Kroneis, Shukun Chen, and Amin El-Heliebi -- Frank Coumans and Leon Terstappen.
This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques throughout recent years including general considerations on bias in WGA, possible sample pre-enrichment strategies and how to run a single-cell lab. The second part focuses on major WGA methods and protocols that allow the assessment of WGA product quality. The final chapters contain advanced protocols and address issues such as sample preparation using laser-micro dissection; WGA from partially degraded DNA (formalin-fixed paraffin embedded samples); circulating tumor cells; and ancient samples.--
9781493929894 1493929895
2015945590
101676379 DNLM
Gene amplification--Laboratory manuals.
Genomics--Laboratory manuals.
Gene Amplification.
Genomics.
Gene amplification.
Genomics.
Laboratory Manuals.
Laboratory manuals.
QH450.3 / .W46 2015
571
W1 / ME9616J v.1347 2015 QU 25
Includes bibliographical references and index.
Principles of whole-genome amplification / Bias in whole genome amplification : causes and considerations / Single-cell lab or how to perform single-cell molecular analysis / Sample preparation methods following cellsearch approach compatible of single-cell whole-genome amplification : an overview / Deterministic whole-genome amplification of single cells / Construction of a DNA library on microbeads using whole genome amplification / Heat-induced fragmentation and adapter-assisted whole genome amplification using GenomePlex® single-cell whole genome amplification kit (WGA4) / Whole genome amplification by isothermal multiple strand displacement using Phi29 DNA polymerase / Using multiplex PCR for assessing the quality of whole genome amplified DNA / Quality control of isothermal amplified DNA based on short tandem repeat analysis / Laser microdissection of FFPE tissue areas and subsequent whole genome amplification by Ampli1 / Whole genome amplification from blood spot samples / Analysis of whole mitogenomes from ancient samples / Copy number variation analysis by array analysis of single cells following whole genome amplification / Whole genome amplification in genomic analysis of single circulating tumor cells / Whole genome amplification of labeled viable single cells suited for array-comparative genomic hybridization / Low-volume on-chip single-cell whole genome amplification for multiple subsequent analyses / Detection and characterization of circulating tumor cells by the cellsearch approach / Zbigniew Tadeusz Czyz, Stefan Kirsch, and Bernhard Polzer -- Jeffrey Sabina and John H. Leamon -- Roland Kirchner and Marianna Alunni-Fabbroni -- Joost F. Swennenhuis and Leon Terstappen -- Zbigniew Tadeusz Czyż and Christoph A. Klein -- Takaaki Kojima, Bo Zhu, and Hideo Nakano -- Amin El-Heliebi, Shukun Chen, and Thomas Kroneis -- Thomas Kroneis and Amin El-Heliebi -- Amin El-Heliebi, Shukun Chen, and Thomas Kroneis -- Thomas Kroneis and Amin El-Heliebi -- Zbigniew Tadeusz Czyz, Nikolas H. Stoecklein, and Bernhard Polzer -- Karina Meden Sørensen -- Gloria Gonzales Fortes and Johanna L.A. Paijmans -- Eftychia Dimitriadou, Masoud Zamani Esteki, and Joris Robert Vermeesch -- Christin Gasch, Klaus Pantel, and Sabine Riethdorf -- Thomas Kroneis and Amin El-Heliebi -- Thomas Kroneis, Shukun Chen, and Amin El-Heliebi -- Frank Coumans and Leon Terstappen.
This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques throughout recent years including general considerations on bias in WGA, possible sample pre-enrichment strategies and how to run a single-cell lab. The second part focuses on major WGA methods and protocols that allow the assessment of WGA product quality. The final chapters contain advanced protocols and address issues such as sample preparation using laser-micro dissection; WGA from partially degraded DNA (formalin-fixed paraffin embedded samples); circulating tumor cells; and ancient samples.--
9781493929894 1493929895
2015945590
101676379 DNLM
Gene amplification--Laboratory manuals.
Genomics--Laboratory manuals.
Gene Amplification.
Genomics.
Gene amplification.
Genomics.
Laboratory Manuals.
Laboratory manuals.
QH450.3 / .W46 2015
571
W1 / ME9616J v.1347 2015 QU 25