CRISPR: methods and protocols / (Record no. 6993)

MARC details
000 -LEADER
fixed length control field 04479cam a22004697i 4500
001 - CONTROL NUMBER
control field 18551934
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20220527082420.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 150402s2015 nyua b 001 0 eng
010 ## - LIBRARY OF CONGRESS CONTROL NUMBER
LC control number 2015937760
016 7# - NATIONAL BIBLIOGRAPHIC AGENCY CONTROL NUMBER
Record control number 101666839
Source DNLM
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 9781493926862 (hbk : alk. paper)
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 1493926861 (hbk : alk. paper)
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
Canceled/invalid ISBN 9781493926879 (eBook)
035 ## - SYSTEM CONTROL NUMBER
System control number (OCoLC)ocn904811957
040 ## - CATALOGING SOURCE
Original cataloging agency NLM
Language of cataloging eng
Transcribing agency NLM
Description conventions rda
Modifying agency YDXCP
-- BTCTA
-- CTB
-- CDX
-- OCLCF
-- IAL
-- OCLCO
-- DLC
042 ## - AUTHENTICATION CODE
Authentication code nlmcopyc
-- lccopycat
050 00 - LIBRARY OF CONGRESS CALL NUMBER
Classification number QH450
Item number .C74 2015
060 00 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER
Classification number W1
Item number ME9616J v.1311 2015
060 10 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER
Classification number QU 25
082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 575
Edition number 22
245 00 - TITLE STATEMENT
Title CRISPR: methods and protocols /
Statement of responsibility, etc. edited by Magnus Lundgren, Emmanuelle Charpentier, Peter C. Fineran.
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. New York
Name of publisher, distributor, etc. Humana Press
Date of publication, distribution, etc. 2015
300 ## - PHYSICAL DESCRIPTION
Extent xiv, 366 pages :
Other physical details illustrations ;
Dimensions 27 cm.
490 1# - SERIES STATEMENT
Series statement Methods in molecular biology,
International Standard Serial Number 1064-3745 ;
Volume/sequential designation 1311
490 1# - SERIES STATEMENT
Series statement Springer protocols,
International Standard Serial Number 1949-2448
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Includes bibliographical references and index (pages 362-366).
505 0# - FORMATTED CONTENTS NOTE
Formatted contents note Investigating CRISPR RNA Biogenesis and Function using RNA-seq -- In vitro Co-reconstitution of Cas Protein Complexes -- Analysis of CRISPR pre-crRNA Cleavage -- Annotation and Classification of CRISPR-Cas Systems -- Computational Detection of CRISPR/crRNA Targets -- High-throughput CRISPR typing of Mycobacterium tuberculosis Complex and Salmonella enterica Serotype Typhimurium -- Spacer-based Macroarrays for CRISPR Genotyping -- Analysis of crRNA using Liquid Chromatography Electrospray Ionisation Mass Spectrometry (LC ESI MS) -- Rapid Multiplex Creation of Escherichia coli Strains Capable of Interfering with Phage Infection through CRISPR -- Exploring CRISPR Interference by Transformation with Plasmid Mixtures: Identification Of Target Interference Motifs in Escherichia coli -- Electrophoretic Mobility Shift Assay of DNA and CRISPR-Cas Ribonucleoprotein Complexes -- Expression and Purification of the CMR (Type III-B) Complex in Sulfolobus solfataricus -- Procedures for Generating CRISPR Mutants With Novel Spacers Acquired from Viruses Or Plasmids -- Archaeal Viruses of the Sulfolobales: Isolation, Infection, and CRISPR Spacer Acquisition -- Using the CRISPR-Cas system to Positively Select Mutants In Genes Essential for its Functionl -- Analysis of Nuclease Activity of Cas1 Proteins Against Complex DNA Substrates -- Characterizing Metal-Dependent Nucleases of CRISPR-Cas prokaryotic Adaptive Immunity Systems -- Cas3 Nuclease-Helicase Activity Assays -- Chemical and Enzymatic Footprint Analyses of R-loop Formation by Cascade-crRNA Complex -- Creation and Analysis of a Virome: Using CRISPR Spacers -- Targeted Mutagenesis in Zebrafish using CRISPR RNA-guided Nucleases -- Precise Genome Editing of Drosophila with CRISPR RNA-guided Cas9 -- Targeted Transcriptional Repression in Bacteria using CRISPR Interference (CRISPRi).
520 ## - SUMMARY, ETC.
Summary, etc. This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems--
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Genetic regulation.
9 (RLIN) 15698
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Bacterial genetics.
650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CRISPR-Associated Proteins
General subdivision analysis
Form subdivision Laboratory Manuals.
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Bacterial genetics.
Source of heading or term fast
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Genetic regulation.
Source of heading or term fast
9 (RLIN) 15698
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Lundgren, Magnus,
Relator term editor.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Charpentier, Emmanuelle,
Dates associated with a name 1968-
Relator term editor.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Fineran, Peter C.,
Relator term editor.
830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE
Uniform title Methods in molecular biology (Clifton, N.J.) ;
Volume/sequential designation 1311.
830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE
Uniform title Springer protocols (Series)
9 (RLIN) 8945
906 ## - LOCAL DATA ELEMENT F, LDF (RLIN)
a 7
b cbc
c copycat
d 2
e ncip
f 20
g y-gencatlg
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Dewey Decimal Classification
Koha item type Books
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Source of acquisition Total Checkouts Full call number Barcode Date last seen Date last checked out Price effective from Koha item type
    Dewey Decimal Classification   Not For Loan Non-fiction Tan Tao University Tan Tao University General Stacks 09/09/2016 Buy of Fahasa 1 575 A-2016-0239 12/16/2019 12/16/2019 09/09/2016 Books

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