MARC details
000 -LEADER |
fixed length control field |
04479cam a22004697i 4500 |
001 - CONTROL NUMBER |
control field |
18551934 |
005 - DATE AND TIME OF LATEST TRANSACTION |
control field |
20220527082420.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
150402s2015 nyua b 001 0 eng |
010 ## - LIBRARY OF CONGRESS CONTROL NUMBER |
LC control number |
2015937760 |
016 7# - NATIONAL BIBLIOGRAPHIC AGENCY CONTROL NUMBER |
Record control number |
101666839 |
Source |
DNLM |
020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
International Standard Book Number |
9781493926862 (hbk : alk. paper) |
020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
International Standard Book Number |
1493926861 (hbk : alk. paper) |
020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
Canceled/invalid ISBN |
9781493926879 (eBook) |
035 ## - SYSTEM CONTROL NUMBER |
System control number |
(OCoLC)ocn904811957 |
040 ## - CATALOGING SOURCE |
Original cataloging agency |
NLM |
Language of cataloging |
eng |
Transcribing agency |
NLM |
Description conventions |
rda |
Modifying agency |
YDXCP |
-- |
BTCTA |
-- |
CTB |
-- |
CDX |
-- |
OCLCF |
-- |
IAL |
-- |
OCLCO |
-- |
DLC |
042 ## - AUTHENTICATION CODE |
Authentication code |
nlmcopyc |
-- |
lccopycat |
050 00 - LIBRARY OF CONGRESS CALL NUMBER |
Classification number |
QH450 |
Item number |
.C74 2015 |
060 00 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER |
Classification number |
W1 |
Item number |
ME9616J v.1311 2015 |
060 10 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER |
Classification number |
QU 25 |
082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
Classification number |
575 |
Edition number |
22 |
245 00 - TITLE STATEMENT |
Title |
CRISPR: methods and protocols / |
Statement of responsibility, etc. |
edited by Magnus Lundgren, Emmanuelle Charpentier, Peter C. Fineran. |
260 ## - PUBLICATION, DISTRIBUTION, ETC. |
Place of publication, distribution, etc. |
New York |
Name of publisher, distributor, etc. |
Humana Press |
Date of publication, distribution, etc. |
2015 |
300 ## - PHYSICAL DESCRIPTION |
Extent |
xiv, 366 pages : |
Other physical details |
illustrations ; |
Dimensions |
27 cm. |
490 1# - SERIES STATEMENT |
Series statement |
Methods in molecular biology, |
International Standard Serial Number |
1064-3745 ; |
Volume/sequential designation |
1311 |
490 1# - SERIES STATEMENT |
Series statement |
Springer protocols, |
International Standard Serial Number |
1949-2448 |
504 ## - BIBLIOGRAPHY, ETC. NOTE |
Bibliography, etc. note |
Includes bibliographical references and index (pages 362-366). |
505 0# - FORMATTED CONTENTS NOTE |
Formatted contents note |
Investigating CRISPR RNA Biogenesis and Function using RNA-seq -- In vitro Co-reconstitution of Cas Protein Complexes -- Analysis of CRISPR pre-crRNA Cleavage -- Annotation and Classification of CRISPR-Cas Systems -- Computational Detection of CRISPR/crRNA Targets -- High-throughput CRISPR typing of Mycobacterium tuberculosis Complex and Salmonella enterica Serotype Typhimurium -- Spacer-based Macroarrays for CRISPR Genotyping -- Analysis of crRNA using Liquid Chromatography Electrospray Ionisation Mass Spectrometry (LC ESI MS) -- Rapid Multiplex Creation of Escherichia coli Strains Capable of Interfering with Phage Infection through CRISPR -- Exploring CRISPR Interference by Transformation with Plasmid Mixtures: Identification Of Target Interference Motifs in Escherichia coli -- Electrophoretic Mobility Shift Assay of DNA and CRISPR-Cas Ribonucleoprotein Complexes -- Expression and Purification of the CMR (Type III-B) Complex in Sulfolobus solfataricus -- Procedures for Generating CRISPR Mutants With Novel Spacers Acquired from Viruses Or Plasmids -- Archaeal Viruses of the Sulfolobales: Isolation, Infection, and CRISPR Spacer Acquisition -- Using the CRISPR-Cas system to Positively Select Mutants In Genes Essential for its Functionl -- Analysis of Nuclease Activity of Cas1 Proteins Against Complex DNA Substrates -- Characterizing Metal-Dependent Nucleases of CRISPR-Cas prokaryotic Adaptive Immunity Systems -- Cas3 Nuclease-Helicase Activity Assays -- Chemical and Enzymatic Footprint Analyses of R-loop Formation by Cascade-crRNA Complex -- Creation and Analysis of a Virome: Using CRISPR Spacers -- Targeted Mutagenesis in Zebrafish using CRISPR RNA-guided Nucleases -- Precise Genome Editing of Drosophila with CRISPR RNA-guided Cas9 -- Targeted Transcriptional Repression in Bacteria using CRISPR Interference (CRISPRi). |
520 ## - SUMMARY, ETC. |
Summary, etc. |
This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems-- |
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name entry element |
Genetic regulation. |
9 (RLIN) |
15698 |
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name entry element |
Bacterial genetics. |
650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name entry element |
CRISPR-Associated Proteins |
General subdivision |
analysis |
Form subdivision |
Laboratory Manuals. |
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name entry element |
Bacterial genetics. |
Source of heading or term |
fast |
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name entry element |
Genetic regulation. |
Source of heading or term |
fast |
9 (RLIN) |
15698 |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Lundgren, Magnus, |
Relator term |
editor. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Charpentier, Emmanuelle, |
Dates associated with a name |
1968- |
Relator term |
editor. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Fineran, Peter C., |
Relator term |
editor. |
830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE |
Uniform title |
Methods in molecular biology (Clifton, N.J.) ; |
Volume/sequential designation |
1311. |
830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE |
Uniform title |
Springer protocols (Series) |
9 (RLIN) |
8945 |
906 ## - LOCAL DATA ELEMENT F, LDF (RLIN) |
a |
7 |
b |
cbc |
c |
copycat |
d |
2 |
e |
ncip |
f |
20 |
g |
y-gencatlg |
942 ## - ADDED ENTRY ELEMENTS (KOHA) |
Source of classification or shelving scheme |
Dewey Decimal Classification |
Koha item type |
Books |