Human embryonic stem cell protocols / edited by Kursad Turksen.

Includes bibliographical references and index.

Human embryo culture and assessment for the derivation of embryonic stem cells (ESC) / A. Henry Sathananthan and Tiki Osianlis -- Derivation of human embryonic stem cell lines from vitrified human embryos / Teija T. Peura, Julia Schaft, and Tomas Stojanov -- Growth of human embryonic stem cells using derivates of human fibroblasts / Carmen Escobedo-Lucea and miodrag Stojkovic -- In vitro neural differentiation of human embronic stem cells using a low-density mouse embryonic fibroblast feeder protocol / John A. Ozolek ... [et al.] -- Optimization of physiological xenofree molecularly defined media and matrices to maintain human embryonic stem cell pluripotency / Isabelle Pfeiffer ... [et al.] -- Serum-free and feeder-free culture expansion of human embryonic stem cells / Katherine E. Wagner and Mohan C. Vemuri -- Single cell enzymatic dissociation of human embryonic stem cells : a straightforward, robust, and standardized culture method / Catharina Ellerström, Johan Hyllner, and Raimund Strehl -- Monitoring stemness in long-term hESC cultures by real-time PCR / Amparo Galán and Carlos Simón -- Culture and preparation of human embryonic stem cells for proteomics-based applications / Charles C. King -- A two- and three-dimensional approach for visualizing human embryonic stem cell differentiation / Christian B. Brøchner ... [et al.] -- Immunofluorescence and mRNA analysis of human embryonic stem cells (hESCs) grown under feeder-free conditions / Aashir Awan ... [et al.] -- Study of gap junctions in human embryonic stem cells / Raymond C.B. Wong and Alice Pébay -- hESC engineering by integrase-mediated chromosomal targeting / Ying Liu ... [et al.] -- Human embryonic stem cell differentiation on periodontal ligament fibroblasts / Y. Murat Elc̦in, Bülend İnanc̦, and A. Eser Elc̦in -- Generation of neural crest cells and peripheral sensory neurons from human embryonic stem cells / Ronald S. Goldstein ... [et al.] -- Embryonic stem cells as a model for studying melanocyte development / Susan E. Zabierowski and Meenhard Herlyn -- In vitro derivation of chondrogenic cells from human embryonic stem cells / Wei Seong Toh ... [et al.] -- Vascular differentiation of human embryonic stem cells in bioactive hydrogel-based scaffolds / Sharon Gerecht, Lino S. Ferreira, and Robert Langer -- Differentiation of neural precursors and dopaminergic neurons from human embryonic stem cells / Xiao-Quing Zhang and Su-Chung Zhang -- Transplantation of human embryonic stem cells and derivatives to the chick embryo / Ronald S. Goldstein -- Genetic manipulation of human embryonic stem cells / Silvina Epsztejn-Litman and Rachel Eiges -- Genetic manipulation of human embryonic stem cells in serum and feeder-free media / Stefan R. Braam, Chris Denning, and Christine L. Mummery -- Human-induced pluripotent stem cells : derivation, propagation, and freezing in serum- and feeder layer-free culture conditions / Hossein Baharvand ... [et al.]

Also issued online.