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001			18551934
005			20220527082420.0
008			150402s2015 nyua b 001 0 eng
010			_a 2015937760
016	7		_a101666839 _2DNLM
020			_a9781493926862 (hbk : alk. paper)
020			_a1493926861 (hbk : alk. paper)
020			_z9781493926879 (eBook)
035			_a(OCoLC)ocn904811957
040			_aNLM _beng _cNLM _erda _dYDXCP _dBTCTA _dCTB _dCDX _dOCLCF _dIAL _dOCLCO _dDLC
042			_anlmcopyc _alccopycat
050	0	0	_aQH450 _b.C74 2015
060	0	0	_aW1 _bME9616J v.1311 2015
060	1	0	_aQU 25
082	0	4	_a575 _222
245	0	0	_aCRISPR: methods and protocols / _cedited by Magnus Lundgren, Emmanuelle Charpentier, Peter C. Fineran.
260			_aNew York _bHumana Press _c2015
300			_axiv, 366 pages : _billustrations ; _c27 cm.
490	1		_aMethods in molecular biology, _x1064-3745 ; _v1311
490	1		_aSpringer protocols, _x1949-2448
504			_aIncludes bibliographical references and index (pages 362-366).
505	0		_aInvestigating CRISPR RNA Biogenesis and Function using RNA-seq -- In vitro Co-reconstitution of Cas Protein Complexes -- Analysis of CRISPR pre-crRNA Cleavage -- Annotation and Classification of CRISPR-Cas Systems -- Computational Detection of CRISPR/crRNA Targets -- High-throughput CRISPR typing of Mycobacterium tuberculosis Complex and Salmonella enterica Serotype Typhimurium -- Spacer-based Macroarrays for CRISPR Genotyping -- Analysis of crRNA using Liquid Chromatography Electrospray Ionisation Mass Spectrometry (LC ESI MS) -- Rapid Multiplex Creation of Escherichia coli Strains Capable of Interfering with Phage Infection through CRISPR -- Exploring CRISPR Interference by Transformation with Plasmid Mixtures: Identification Of Target Interference Motifs in Escherichia coli -- Electrophoretic Mobility Shift Assay of DNA and CRISPR-Cas Ribonucleoprotein Complexes -- Expression and Purification of the CMR (Type III-B) Complex in Sulfolobus solfataricus -- Procedures for Generating CRISPR Mutants With Novel Spacers Acquired from Viruses Or Plasmids -- Archaeal Viruses of the Sulfolobales: Isolation, Infection, and CRISPR Spacer Acquisition -- Using the CRISPR-Cas system to Positively Select Mutants In Genes Essential for its Functionl -- Analysis of Nuclease Activity of Cas1 Proteins Against Complex DNA Substrates -- Characterizing Metal-Dependent Nucleases of CRISPR-Cas prokaryotic Adaptive Immunity Systems -- Cas3 Nuclease-Helicase Activity Assays -- Chemical and Enzymatic Footprint Analyses of R-loop Formation by Cascade-crRNA Complex -- Creation and Analysis of a Virome: Using CRISPR Spacers -- Targeted Mutagenesis in Zebrafish using CRISPR RNA-guided Nucleases -- Precise Genome Editing of Drosophila with CRISPR RNA-guided Cas9 -- Targeted Transcriptional Repression in Bacteria using CRISPR Interference (CRISPRi).
520			_aThis volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems--
650		0	_aGenetic regulation. _915698
650		0	_aBacterial genetics.
650	1	2	_aCRISPR-Associated Proteins _xanalysis _vLaboratory Manuals.
650		7	_aBacterial genetics. _2fast
650		7	_aGenetic regulation. _2fast _915698
700	1		_aLundgren, Magnus, _eeditor.
700	1		_aCharpentier, Emmanuelle, _d1968- _eeditor.
700	1		_aFineran, Peter C., _eeditor.
830		0	_aMethods in molecular biology (Clifton, N.J.) ; _v1311.
830		0	_aSpringer protocols (Series) _98945
906			_a7 _bcbc _ccopycat _d2 _encip _f20 _gy-gencatlg
942			_2ddc _cBK
999			_c6993 _d6993