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020			_a9781493929894
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020			_z9781493929900
035			_a(OCoLC)ocn909327447
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050	0	0	_aQH450.3 _b.W46 2015
060	0	0	_aW1 _bME9616J v.1347 2015
060	1	0	_aQU 25
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245	0	0	_aWhole genome amplification: methods and protocols / _cedited by Thomas Kroneis, Institute of Cell Biology, Histology & Embryology, Medical University of Graz, Graz, Austria.
260			_aNew York _bHumana Press _c2015
300			_ax, 284 pages : _billustrations (some color) ; _c27 cm.
490	1		_aSpringer protocols, _x1949-2448
490	1		_aMethods in molecular biology, _x1064-3745 ; _vv. 1347
504			_aIncludes bibliographical references and index.
505	0	0	_tPrinciples of whole-genome amplification / _rZbigniew Tadeusz Czyz, Stefan Kirsch, and Bernhard Polzer -- _tBias in whole genome amplification : causes and considerations / _rJeffrey Sabina and John H. Leamon -- _tSingle-cell lab or how to perform single-cell molecular analysis / _rRoland Kirchner and Marianna Alunni-Fabbroni -- _tSample preparation methods following cellsearch approach compatible of single-cell whole-genome amplification : an overview / _rJoost F. Swennenhuis and Leon Terstappen -- _tDeterministic whole-genome amplification of single cells / _rZbigniew Tadeusz Czyż and Christoph A. Klein -- _tConstruction of a DNA library on microbeads using whole genome amplification / _rTakaaki Kojima, Bo Zhu, and Hideo Nakano -- _tHeat-induced fragmentation and adapter-assisted whole genome amplification using GenomePlex® single-cell whole genome amplification kit (WGA4) / _rAmin El-Heliebi, Shukun Chen, and Thomas Kroneis -- _tWhole genome amplification by isothermal multiple strand displacement using Phi29 DNA polymerase / _rThomas Kroneis and Amin El-Heliebi -- _tUsing multiplex PCR for assessing the quality of whole genome amplified DNA / _rAmin El-Heliebi, Shukun Chen, and Thomas Kroneis -- _tQuality control of isothermal amplified DNA based on short tandem repeat analysis / _rThomas Kroneis and Amin El-Heliebi -- _tLaser microdissection of FFPE tissue areas and subsequent whole genome amplification by Ampli1 / _rZbigniew Tadeusz Czyz, Nikolas H. Stoecklein, and Bernhard Polzer -- _tWhole genome amplification from blood spot samples / _rKarina Meden Sørensen -- _tAnalysis of whole mitogenomes from ancient samples / _rGloria Gonzales Fortes and Johanna L.A. Paijmans -- _tCopy number variation analysis by array analysis of single cells following whole genome amplification / _rEftychia Dimitriadou, Masoud Zamani Esteki, and Joris Robert Vermeesch -- _tWhole genome amplification in genomic analysis of single circulating tumor cells / _rChristin Gasch, Klaus Pantel, and Sabine Riethdorf -- _tWhole genome amplification of labeled viable single cells suited for array-comparative genomic hybridization / _rThomas Kroneis and Amin El-Heliebi -- _tLow-volume on-chip single-cell whole genome amplification for multiple subsequent analyses / _rThomas Kroneis, Shukun Chen, and Amin El-Heliebi -- _tDetection and characterization of circulating tumor cells by the cellsearch approach / _rFrank Coumans and Leon Terstappen.
520	8		_aThis volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques throughout recent years including general considerations on bias in WGA, possible sample pre-enrichment strategies and how to run a single-cell lab. The second part focuses on major WGA methods and protocols that allow the assessment of WGA product quality. The final chapters contain advanced protocols and address issues such as sample preparation using laser-micro dissection; WGA from partially degraded DNA (formalin-fixed paraffin embedded samples); circulating tumor cells; and ancient samples.--
650		0	_aGene amplification _vLaboratory manuals.
650		0	_aGenomics _vLaboratory manuals.
650	1	2	_aGene Amplification.
650	2	2	_aGenomics.
650		7	_aGene amplification. _2fast
650		7	_aGenomics. _2fast
655		2	_aLaboratory Manuals.
655		7	_aLaboratory manuals. _2fast
700	1		_aKroneis, Thomas, _eeditor.
830		0	_aSpringer protocols (Series), _98945
830		0	_aMethods in molecular biology (Clifton, N.J.) ; _vv. 1347,
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