Whole genome amplification: methods and protocols / edited by Thomas Kroneis, Institute of Cell Biology, Histology & Embryology, Medical University of Graz, Graz, Austria.

Includes bibliographical references and index.

Principles of whole-genome amplification / Zbigniew Tadeusz Czyz, Stefan Kirsch, and Bernhard Polzer -- Bias in whole genome amplification : causes and considerations / Jeffrey Sabina and John H. Leamon -- Single-cell lab or how to perform single-cell molecular analysis / Roland Kirchner and Marianna Alunni-Fabbroni -- Sample preparation methods following cellsearch approach compatible of single-cell whole-genome amplification : an overview / Joost F. Swennenhuis and Leon Terstappen -- Deterministic whole-genome amplification of single cells / Zbigniew Tadeusz Czyż and Christoph A. Klein -- Construction of a DNA library on microbeads using whole genome amplification / Takaaki Kojima, Bo Zhu, and Hideo Nakano -- Heat-induced fragmentation and adapter-assisted whole genome amplification using GenomePlex® single-cell whole genome amplification kit (WGA4) / Amin El-Heliebi, Shukun Chen, and Thomas Kroneis -- Whole genome amplification by isothermal multiple strand displacement using Phi29 DNA polymerase / Thomas Kroneis and Amin El-Heliebi -- Using multiplex PCR for assessing the quality of whole genome amplified DNA / Amin El-Heliebi, Shukun Chen, and Thomas Kroneis -- Quality control of isothermal amplified DNA based on short tandem repeat analysis / Thomas Kroneis and Amin El-Heliebi -- Laser microdissection of FFPE tissue areas and subsequent whole genome amplification by Ampli1 / Zbigniew Tadeusz Czyz, Nikolas H. Stoecklein, and Bernhard Polzer -- Whole genome amplification from blood spot samples / Karina Meden Sørensen -- Analysis of whole mitogenomes from ancient samples / Gloria Gonzales Fortes and Johanna L.A. Paijmans -- Copy number variation analysis by array analysis of single cells following whole genome amplification / Eftychia Dimitriadou, Masoud Zamani Esteki, and Joris Robert Vermeesch -- Whole genome amplification in genomic analysis of single circulating tumor cells / Christin Gasch, Klaus Pantel, and Sabine Riethdorf -- Whole genome amplification of labeled viable single cells suited for array-comparative genomic hybridization / Thomas Kroneis and Amin El-Heliebi -- Low-volume on-chip single-cell whole genome amplification for multiple subsequent analyses / Thomas Kroneis, Shukun Chen, and Amin El-Heliebi -- Detection and characterization of circulating tumor cells by the cellsearch approach / Frank Coumans and Leon Terstappen.

This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques throughout recent years including general considerations on bias in WGA, possible sample pre-enrichment strategies and how to run a single-cell lab. The second part focuses on major WGA methods and protocols that allow the assessment of WGA product quality. The final chapters contain advanced protocols and address issues such as sample preparation using laser-micro dissection; WGA from partially degraded DNA (formalin-fixed paraffin embedded samples); circulating tumor cells; and ancient samples.--